Journal: Oncology Letters

Article Title: Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene

doi: 10.3892/ol.2017.6248

Figure Lengend Snippet: Treatment with EGCG leads to increased p16 mRNA expression. (A) Results of the agarose gel electrophoresis. (B) Quantitated p16 mRNA expression levels relative to GAPDH. **P<0.01 vs. the untreated cells. EGCG, epigallocatechin-3-gallate; p16, cyclin-dependent kinase inhibitor 2A.

Article Snippet: GAPDH was blotted as an internal reference using the anti-GAPDH rabbit polyclonal antibody (cat. no. AB21612-1; 1:5,000; AbSci, LLC, Portland, OR, USA).

Techniques: Expressing, Agarose Gel Electrophoresis

Journal: Leukemia Research Reports

Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line

doi: 10.1016/j.lrr.2016.08.001

Figure Lengend Snippet: The sequences of primers used to amplify MDR1, BSG, BIRC5 and GAPDH mRNAs [3] , [17] .

Article Snippet: This study used the following antibodies: mouse anti-CD147 monoclonal antibody clone MEM-M6/6 (Exbio, Vestec, Czech Republic); mouse anti-P-gp monoclonal antibody (MT PGP1) (Biomedical Technology Research Center, Chiang Mai, Thailand) [ ], mouse anti-γ 4 globin monoclonal antibody (PB1) (Biomedical Technology Research Center) [ ] , rabbit anti-CD147 polyclonal antibody (Abcam, Cambridge, UK) , rabbit anti-GAPDH polyclonal antibody (Merck Millipore, Billeric, MA, USA), goat anti-rabbit IgG-HRP (Abcam), and rabbit anti-mouse Igs-FITC (Dako, Hamburg, Germany).

Techniques: Sequencing

Journal: Leukemia Research Reports

Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line

doi: 10.1016/j.lrr.2016.08.001

Figure Lengend Snippet: The protein expressions of P-gp and CD147 in drug-resistant K562/ADR and drug-free K562/ADR leukemic cell lines . Proteins were extracted drug-resistant K562/ADR and drug-free K562/ADR cell lines and separated by 12% SDS-PAGE and transferred onto PVDF membrane. The membranes were probed with anti-CD147, anti-P-gp and detected by enhanced chemiluminescence. (A) Results are representative of three independent experiments by Western blotting and (B) Quantification of P-gp and CD147 expressions in drug-resistant K562/ADR and drug-free K562/ADR cells; the protein levels were normalized to the loading control GAPDH. The ratio of P-gp and CD147 were calculated for each group. Data are presented as mean±SEM of three independent experiments; ⁎ p <0.05 when compared to drug-free K562/ADR cell.

Article Snippet: This study used the following antibodies: mouse anti-CD147 monoclonal antibody clone MEM-M6/6 (Exbio, Vestec, Czech Republic); mouse anti-P-gp monoclonal antibody (MT PGP1) (Biomedical Technology Research Center, Chiang Mai, Thailand) [ ], mouse anti-γ 4 globin monoclonal antibody (PB1) (Biomedical Technology Research Center) [ ] , rabbit anti-CD147 polyclonal antibody (Abcam, Cambridge, UK) , rabbit anti-GAPDH polyclonal antibody (Merck Millipore, Billeric, MA, USA), goat anti-rabbit IgG-HRP (Abcam), and rabbit anti-mouse Igs-FITC (Dako, Hamburg, Germany).

Techniques: SDS Page, Membrane, Western Blot, Control

Journal: Leukemia Research Reports

Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line

doi: 10.1016/j.lrr.2016.08.001

Figure Lengend Snippet: The level of MDR1 and BSG mRNA in drug-resistant K562/ADR and drug-free K562/ADR leukemic cell lines. Total RNA was extracted from cells and the reverse transcription reaction were performed using a RevertAid First Strand cDNA Synthesis kit (Thermo Scientific™). The newly synthesized cDNA was amplified by PCR using specific primers. The PCR product were electrophoresed on 1.2% agarose gel and visualized by ethidium bromide staining. (A) Results are representative of three independent experiments by semiquantitative RT-PCR analysis and (B) quantification of MDR1 (multidrug resistance 1) and BSG (Basigin) mRNA expression in drug-resistant K562/ADR and drug-free K562/ADR cells; the mRNA levels were normalized to the loading control GAPDH. The ratio of MDR1 and BSG mRNAs were calculated for each group. Data are presented as mean ±SEM of three independent experiments; ⁎ p <0.05 when compared to drug-free K562/ADR cell.

Article Snippet: This study used the following antibodies: mouse anti-CD147 monoclonal antibody clone MEM-M6/6 (Exbio, Vestec, Czech Republic); mouse anti-P-gp monoclonal antibody (MT PGP1) (Biomedical Technology Research Center, Chiang Mai, Thailand) [ ], mouse anti-γ 4 globin monoclonal antibody (PB1) (Biomedical Technology Research Center) [ ] , rabbit anti-CD147 polyclonal antibody (Abcam, Cambridge, UK) , rabbit anti-GAPDH polyclonal antibody (Merck Millipore, Billeric, MA, USA), goat anti-rabbit IgG-HRP (Abcam), and rabbit anti-mouse Igs-FITC (Dako, Hamburg, Germany).

Techniques: Reverse Transcription, cDNA Synthesis, Synthesized, Amplification, Agarose Gel Electrophoresis, Staining, Reverse Transcription Polymerase Chain Reaction, Expressing, Control

Journal: Leukemia Research Reports

Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line

doi: 10.1016/j.lrr.2016.08.001

Figure Lengend Snippet: Effect of MEM-M6/6 anti-CD147 on P-gp (A) and CD147 (B) protein expression in drug-resistant K562/ADR cell line. The protein levels after treating drug-resistant K562/ADR cell with (1) 0 μg/mL MEM-M6/6, (2) 0.625 μg/mL, (3) 1.25 μg/mL, (4) 2.5 μg/mL, and (5) 5 μg/mL for 48 h were determined by Western blotting. The bands were quantified using a scan densitometer. The protein levels were normalized to the loading control GAPDH. Data are presented as mean±SEM of three independent experiments; ⁎ p <0.05 when compared to untreated cell.

Article Snippet: This study used the following antibodies: mouse anti-CD147 monoclonal antibody clone MEM-M6/6 (Exbio, Vestec, Czech Republic); mouse anti-P-gp monoclonal antibody (MT PGP1) (Biomedical Technology Research Center, Chiang Mai, Thailand) [ ], mouse anti-γ 4 globin monoclonal antibody (PB1) (Biomedical Technology Research Center) [ ] , rabbit anti-CD147 polyclonal antibody (Abcam, Cambridge, UK) , rabbit anti-GAPDH polyclonal antibody (Merck Millipore, Billeric, MA, USA), goat anti-rabbit IgG-HRP (Abcam), and rabbit anti-mouse Igs-FITC (Dako, Hamburg, Germany).

Techniques: Expressing, Western Blot, Control

Journal: Leukemia Research Reports

Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line

doi: 10.1016/j.lrr.2016.08.001

Figure Lengend Snippet: Effect of MEM-M6/6 anti-CD147 on MDR1 (A) and BSG (B) mRNA levels in drug-resistant K562/ADR cell line. The quantification of mRNA expression in the presence of various concentrations of MEM-M6/6 (0–5 µg/mL) were normalized to the loading control GAPDH mRNA. The ratio of mRNA was calculated for each group. Data are presented as mean±SEM of three independent experiments; ⁎ p <0.05 when compared to untreated K562/ADR cell.

Article Snippet: This study used the following antibodies: mouse anti-CD147 monoclonal antibody clone MEM-M6/6 (Exbio, Vestec, Czech Republic); mouse anti-P-gp monoclonal antibody (MT PGP1) (Biomedical Technology Research Center, Chiang Mai, Thailand) [ ], mouse anti-γ 4 globin monoclonal antibody (PB1) (Biomedical Technology Research Center) [ ] , rabbit anti-CD147 polyclonal antibody (Abcam, Cambridge, UK) , rabbit anti-GAPDH polyclonal antibody (Merck Millipore, Billeric, MA, USA), goat anti-rabbit IgG-HRP (Abcam), and rabbit anti-mouse Igs-FITC (Dako, Hamburg, Germany).

Techniques: Expressing, Control